Biological Systems: Theory and Innovation

Environmental efficiency of recirculating water supply installations in the artificial breeding of noble crayfish

Artem Kravchenko, Kateryna Bozhko
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Abstract

The aim of the study was to assess the effectiveness of a closed water-cycle technology that reduces the impact on natural water bodies and promotes the rational use of water resources. Experimental, analytical and statistical methods were used in the study: biometric measurements, hydrochemical monitoring, behavioural observations and correlation analysis. At the beginning of the experiment, the mean length of the juveniles was 1.2 cm and the weight 1.8 g, whereas by the end of the observations these indicators had increased to 5.1 cm and 11.7 g respectively. Survival remained within 85-100%, which indicated optimal cultivation conditions. The water temperature was stably maintained at 21-22°C, and the oxygen concentration fluctuated within 6.8-7.1 mg/l, ensuring normal metabolism of hydrobionts. Hydrochemical analysis showed the effective functioning of the biofilter: the concentration of ammonium nitrogen decreased from 0.35 to 0.07 mg/l, nitrites from 0.15 to 0.03 mg/l, and nitrates from 4.8 to 2.8 mg/l. Water transparency increased from 42 to 55 cm, and pH stabilised at 7.5, which indicated completion of the stage of biological stabilisation of the environment. Behavioural indicators also confirmed successful adaptation: activity of the juveniles increased from 2.1 to 4.6 points, the stress level decreased from 3.8 to 1.8 points, and cannibalism fell to 0.9%. After transfer to a natural water body, crayfish survival was 72%, the mean weight 133 g, and the proportion of females with eggs reached 65%, which confirmed the natural reproductive capacity. Economic calculations showed production profitability of 55.2% at a cost price of 138 UAH/kg and a net profit of 2050 UAH/m²/year. Energy consumption of the system was low – 3.6 kWh/day, saving of water resources – 92%, and the environmental effect – a 40% reduction in the catch of natural populations. The practical significance of this study lies in the possibility of using its results for the introduction of energy-efficient technologies for rearing noble crayfish on farms and in industrial enterprises in Ukraine

Keywords

natural populations; survival; hydrobionts; profitability; adaptation

Suggested citation
Kravchenko, A., & Bozhko, K. (2025). Environmental efficiency of recirculating water supply installations in the artificial breeding of noble crayfish. Biological Systems: Theory and Innovation, 16(4), 23-39. https://doi.org/10.31548/biologiya/4.2025.23
References
  1. Agbugui, M.O., Inobeme, A., Okhamafe, E.P., Abhulimen, F.E., Abe, G., Odozie, J.C., & Ogbiti, J.T. (2025). Aquaculture production and management practices of farmed fish in Nigeria. In A. Gupta (Ed.), Prospects of fungal biotechnologies for livestock: Fungal bioengineering in livestock health management (volume 1, pp. 167-189). Cham: Springer. doi: 10.1007/978-3-031-90994-8_7.
  2. Agro-Ukraine. (n.d.). Crayfish in the Kyiv region. Retrieved from https://agro-ukraine.com/ua/trade/rgn-14/r-312/p-2/.
  3. Alvanou, M.V., Papadopoulos, D.K., Lattos, A., Georgoulis, I., Feidantsis, K., Apostolidis, Α.P., Michaelidis, B., & Giantsis, I.A. (2022). Biology, distribution, conservation status and stocking perspective of freshwater crayfish in Greece: An updated review. Aquaculture Research, 53(15), 5115-5128. doi: 10.1111/are.16009.
  4. Araujo, G.S., Silva, J.W, Cotas, J., & Pereira, L. (2022). Fish farming techniques: Current situation and trends. Journal of Marine Science and Engineering, 10(11), article number 1598. doi: 10.3390/jmse10111598.
  5. Bloomer, C.C., Miller, C.M., DiStefano, R.J., & Taylor, C.A. (2024). Midwest prairie management practices benefit the non-target prairie crayfish. Fire Ecology, 20, article number 11. doi: 10.1186/s42408-023-00243-x
  6. Cheng, W.K., Tan, T.B., San Tan, J., Yi-Le Chan, J., Bea, K.T., & Chen, Y.L. (2025). Smart aquaculture: Integrating computer vision and IoT for automated crayfish health assessment via federated learning. IEEE Access, 13, 189979-189994. doi: 10.1109/ACCESS.2025.3624140.
  7. Daniels, J.A., Kerr, J.R., & Kemp, P.S. (2023). River infrastructure and the spread of freshwater invasive species: Inferences from an experimentally‐parameterised individual‐based model. Journal of Applied Ecology, 60(6), 999-1009. doi: 10.1111/1365-2664.14387.
  8. DSTU EN ISO/IEC 17025:2019 “General requirements for the competence of testing and calibration laboratories”. (2019). Retrieved from https://online.budstandart.com/ua/catalog/doc-page.html?id_doc=88724.
  9. Duffy, R.E., Hodgson, B., Quinn, A., & Newman, M. (2025). Successful artificial incubation and juvenile-rearing of dropped eggs of a critically endangered freshwater crayfish (Cherax tenuimanus). Marine and Freshwater Research, 76(12), article number MF25063. doi: 10.1071/MF25063.
  10. Edwards, O.M., Banks, K.M., Zhang, B., & Reichert, M.S. (2025). Population dynamics and environmental drivers of crawfish frog (Rana areolata) migration in Southeastern Oklahoma, USA. Herpetological Conservation Biology, 20(2), 196-212.
  11. Eilitta, M., Nambeye-Kaonga, E., Gordon Mudenda, H., Katapa, C., Chimai-Mulenga, B., & Rice, A. (2023). Population ecology and current distribution assessment of the introduced invasive crayfish in the Kafue Floodplain and Lake Kariba, Zambia. Retrieved from https://www.fishinnovationlab.msstate.edu/research/projects/population-ecology-and-current-distribution-assessment-introduced-invasive.
  12. Emerenciano, M.G., Rombenso, A.N., Vieira, F.D., Martins, M.A., Coman, G.J., Truong, H.H., Noble, T.H., & Simon, C.J. (2022). Intensification of penaeid shrimp culture: An applied review of advances in production systems, nutrition and breeding. Animals, 12(3), article number 236. doi: 10.3390/ani12030236.
  13. European Commission. (2021). Ethics and data protection. Retrieved from https://ec.europa.eu/info/funding-tenders/opportunities/docs/2021-2027/horizon/guidance/ethics-and-data-protection_he_en.pdf.
  14. Greenhalgh, J.A., et al. (2024). Environmental DNA can inform the trade‐off between proactive and reactive strategies for crayfish conservation. Environmental DNA, 6(3), article number e571. doi: 10.1002/edn3.571.
  15. Hapich, H., Novitskyi, R., Onopriienko, D., & Dubov, T. (2024). Water on fire: Losses and the post-war future of ecosystem services from water resources of Ukraine. Regional Environmental Change, 24, article number 154. doi: 10.1007/s10113-024-02320-6.
  16. Haubrock, P.J., Oficialdegui, F.J., Zeng, Y., Patoka, J., Yeo, D.C., & Kouba, A. (2021). The redclaw crayfish: A prominent aquaculture species with invasive potential in tropical and subtropical biodiversity hotspots. Reviews in Aquaculture, 13(3), 1488-1530. doi: 10.1111/raq.12531.
  17. Hinchcliffe, J., Agnalt, A.L., Daniels, C.L., Drengstig, A.R., Lund, I., McMinn, J., & Powell, A. (2022). European lobster Homarus gammarus aquaculture: Technical developments, opportunities and requirements. Reviews in Aquaculture, 14(2), 919-937. doi: 10.1111/raq.12634.
  18. Hrynevych, N., Zharchynska, V., Svitelskyi, M., Khomiak, O., & Sliusarenko, A. (2022). Promising object of aquaculture of crustaceans Cherax quadricarinatus (Von Martens, 1868): Biology, technology (review). Aquatic Biological Resources and Aquaculture, 1, 47-62. doi: 10.32851/wba.2022.1.4.
  19. Hudina, S., Maguire, I., Dragičević, P., & Galic, N. (2022). Evaluating the efficacy of approaches to control invasive populations: A conceptual model development for the signal crayfish. Ecologies, 3(2), 78-95. doi: 10.3390/ecologies3020008.
  20. Ishchuk, O., Svitelskyi, M., Matkovska, S., Sliusar, M., & Kovalchuk, I. (2024). Current status and development trends crustaceans aquaculture. Ukrainian Journal of Natural Sciences, 7, 18-24. doi: 10.32782/naturaljournal.7.2024.2.
  21. Kwikiriza, G., Muthoka, M., Omara, T., Abaho, I., Tibihika, P.D., Curto, M., Opiyo, M.A., Munguti, J., Abwao, J., Orina, P., & Meimberg, H. (2025). Nile tilapia (Oreochromis niloticus L.) cage aquaculture in Africa: Potential threats to congeneric fish species and advances to detect escapes. Aquaculture, Fish and Fisheries, 5(4), article number e70090. doi: 10.1002/aff2.70090.
  22. Latournerié-Cervera, J.R., Estrada-Ortega, A.R., Arana-Magallón, F., & Méndez-Ramírez, I. (2025). Ecophysiological studies of Cambarellus montezumae crayfish from Xochimilco, Mexico: Population phenology and water quality habitat. European Journal of Biology and Biotechnology, 6(2), 6-14. doi: 10.24018/ejbio.2025.6.2.537.
  23. Law of Ukraine No. 249 “On the Procedure for Carrying out Experiments and Experiments on Animals by Scientific Institutions”. (2012, March). Retrieved from https://zakon.rada.gov.ua/laws/show/z0416-12#Text.
  24. Lee, S.S., Unger, A., Teo, S.L., & Shenkar, N. (2025). Culturing the solitary ascidian Phallusia nigra in closed and open water systems for tropical environmental research. Limnology and Oceanography: Methods, 23(10), 700-714. doi: 10.1002/lom3.10713.
  25. Max-Aguilar, A., Villarreal, H., Leyva-Valencia, I., Valencia-Valdez, R., Naranjo-Páramo, J., Vargas-Mendieta, M., Villarreal-Garcia, A., & Cruz-Hernández, P. (2021). Genetic diversity of divergent redclaw crayfish Cherax quadricarinatus (Von Martens, 1868) populations evaluated to initiate a breeding program in Mexico. Latin American Journal of Aquatic Research, 49(2), 272-279. doi: 10.3856/vol49-issue2-fulltext-2630.
  26. McCormack, R.B., & Whiterod, N.S. (2024). Biology, distribution, and conservation of the Gamilaroi crayfish Euastacus gamilaroi Morgan, 1997 (Decapoda: Astacidea: Parastacidae), a freshwater crayfish from New South Wales, Australia. Journal of Crustacean Biology, 44(1), article number ruae009. doi: 10.1093/jcbiol/ruae009.
  27. Mecha, N.J., & Dolorosa, R.G. (2024). Distribution and characteristics of spiny lobster (Panulirus spp.) puerulus collection sites in Palawan, the Philippines. Philippine Journal of Science, 153(3), 1061-1071. doi: 10.56899/153.03.26.
  28. Mohammed, R., Supain, C., Joseph, H., & Kahar, S.A. (2021). Freshwater lobster production-green aquaponics perennial system (FLP-GAPS): A green community-based social enterprise (CBSE) for B40 in Sabah. In R. Hassan, N.H. Abdul Hamid, A.K. Arshad, A. Alisibramulisi, M. Norhasri Muhd Sidek, N. Mohamad Bhkari & E. Shaffie (Eds.), Green infrastructure: Materials and applications (pp. 285-304). Singapore: Springer. doi: 10.1007/978-981-16-6383-3_17.
  29. Oficialdegui, F.J., Bláha, M., Prati, S., Lipták, B., Weiperth, A., Bányai, Z.M., Maciaszek, R., Patoka, J., Scheers, K., Lemmers, P., Petutschnig, J., Petryl, M., Petrusek, A., & Kouba, A. (2025). Contrasting patterns of genetic variability in pet‐traded red swamp crayfish Procambarus clarkii and its feral populations. Freshwater Biology, 70(2), article number e70008. doi: 10.1111/fwb.70008.
  30. Owens, L., & Elliman, J. (2025). Effect of husbandry on the emergence of pathogens in the aquaculture of redclaw crayfish (Cherax quadricarinatus) in Australia. Aquaculture, 603, article number 742380. doi: 10.1016/j.aquaculture.2025.742380.
  31. Qin, L., Guo, C., Xiong, M., Gong, K., Liu, J., Zhang, T., & Li, W. (2023). Effects of shelter on the hatching, immune performance, and profitability of the Ovigerous Red Swamp Crayfish Procambarus clarkii under High Stocking Density. Water, 15(5), article number 907. doi: n10.3390/w15050907.
  32. State Agency for Land Reclamation, Fisheries, and Food Programmes of Ukraine. (n.d.). Industrial fishing of aquatic biological resources. Retrieved from https://darg.gov.ua/_vilov_0_1023_menu_0_1.html.
  33. Terrell, V.C., Maerz, J.C., Engbrecht, N.J., Stiles, R.M., Crawford, B.A., & Lannoo, M.J. (2023). Breeding population dynamics of threatened crawfish frogs inform targets for habitat management. Ichthyology & Herpetology, 111(1), 72-86. doi: 10.1643/h2022031.
  34. Wei, Y., Müller, D., Sun, Z., Lu, M., Tang, H., Wu, W. 2023. Exploring the emergence and changing dynamics of a new integrated rice-crawfish farming system in China. Environmental Research Letters, 18(6), article number 064040. doi: 10.1088/1748-9326/acd8d2.
  35. Wood, R., Coleman, D., McFadden, M., Spark, P., Coote, D., Hill, P., & Furlan, E. (2025). Applying eDNA methods to monitor the distribution of remnant and translocated populations of an endangered amphibian, Litoria booroolongensis. Environmental DNA, 7(5), article number e70208. doi: 10.1002/edn3.70208.