Biological Systems: Theory and Innovation

Regulation of morphometric parameters of winter barley (Hordeum vulgare L.) using graphene oxide under water stress

Tetiana Тkachenko, Yelyzaveta Didur, Olha Melezhyk, Svitlana Prylutska
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Abstract

The problem of food security due to extreme climate change is critical in Europe and globally, and its solution is one of the sustainable development goals, as the amount of precipitation decreases annually, and the average annual temperature increases, which leads to the spread of droughts, lengthening rainless periods, and complicates the cultivation of crops. The purpose of the study was to investigate the effect of structured graphene oxide nanoparticles during pre-sowing treatment of winter barley seeds (Hordeum vulgare L.) varieties ‘Dostoynyi’ of morphometric parameters of plants in conditions of water deficiency. Chemical, physical, and physiological methods were applied. The effect of graphene oxide at concentrations of 5, 10, 20, and 50 micrograms/ml on the morphometric parameters of winter barley was studied 17 days after seed treatment under conditions of regular watering and water stress. No phytotoxic effect of graphene oxide on barley seedlings with regular watering was detected. Pre-sowing treatment of seeds with graphene oxide at a concentration of 5 micrograms/ml showed a maximum increase in root length (by 35%) and plant height (by 23%) with water deficiency compared to the control. At other concentrations studied, plant growth was also stimulated, but the effect was less pronounced. The weight of plants at optimal concentrations of graphene oxide increased slightly, and with water deficiency, the increase was minimal. Graphene oxide effectively improved the development of the barley root system in conditions of water scarcity, which is important for plant adaptation to drought. The practical significance of the study lies in the fact that the results obtained substantiate the feasibility of using nanoparticles as an effective agricultural technology to increase the stress resistance of agricultural crops to water scarcity

Keywords

grain crops; drought resistance; innovative agricultural technologies; nanomaterials; pre-sowing treatment

Suggested citation
Тkachenko, Т., Didur, Ye., Melezhyk, O., & Prylutska, S. (2025). Regulation of morphometric parameters of winter barley (Hordeum vulgare L.) using graphene oxide under water stress. Biological Systems: Theory and Innovation, 16(2), 50-60. https://doi.org/10.31548/biologiya/2.2025.50
References
  1. Anegbe, B., Ifijen, I.H., Maliki, M., Uwidia, I.E., & Aigbodion, A.I. (2024). Graphene oxide synthesis and applications in emerging contaminant removal: A comprehensive review. Environmental Sciences Europe, 36(1), article number 15. doi: 10.1186/s12302-023-00814-4.
  2. Buziashvili, A., Prylutska, S., & Yemets, A. (2024). Effect of fullerene C60 on tomato plants. Innovative Biosystems and Bioengineering, 8(4), 13-22. doi: 10.20535/ibb.2024.8.4.317138.
  3. Convention on Biological Diversity. (1992, June). Retrieved from https://zakon.rada.gov.ua/laws/show/995_030#Text.
  4. Ergun, N., Akdogan, G., Aydogan, S., Bilir, M., & Kilic, G. (2024). Determination of early-stage shoot and root traits of cultivated and wild barley genotypes. Journal of Central European Agriculture, 25(3), 661-674. doi: 10.5513/JCEA01/25.3.4220.
  5. Fathi, A., Shiade, S.R.G., Kianersi, F., Altaf, M.A., Amiri, E., & Nabati, E. (2024). Photosynthesis in cereals under drought stress. In Handbook of photosynthesis (4th ed., pp. 568-576). Boca Raton: CRC Press. doi: 10.1201/b22922-38.
  6. Filipovic, A. (2020). Water plant and soil relation under stress situations. In R.S. Meena & R. Datta (Eds.), Soil moisture importance. IntechOpen. doi: 10.5772/intechopen.93528.
  7. Kompas, T., Che, T.N., & Grafton, R.Q. (2024). Global impacts of heat and water stress on food production and severe food insecurity. Scientific Reports, 14, article number 14398. doi: 10.1038/s41598-024-65274-z.
  8. Lopes, T., Cruz, C., Cardoso, P., Pinto, R., Marques, P.A.A.P., & Figueira, E. (2021). A multifactorial approach to untangle graphene oxide (GO) nanosheets effects on plants: Plant growth-promoting bacteria inoculation, bacterial survival, and drought. Nanomaterials, 11(3), article number 771. doi: 10.3390/nano11030771.
  9. Mahmood, T., et al. (2022). Differential seedling growth and tolerance indices reflect drought tolerance in cotton. BMC Plant Biology, 22, article number 331. doi: 10.1186/s12870-022-03724-4.
  10. Mahmoud, N.E., & Abdelhameed, R.M. (2021). Superiority of modified graphene oxide for enhancing the growth, yield, and antioxidant potential of pearl millet (Pennisetum glaucum L.) under salt stress. Plant Stress, 2, article number 100025. doi: 10.1016/j.stress.2021.100025.
  11. Miranda, M.T., Da Silva, S.F., Silveira, N.M., Pereira, L., Machado, E.C., & Ribeiro, R.V. (2020). Root osmotic adjustment and stomatal control of leaf gas exchange are dependent on citrus rootstocks under water deficit. Journal of Plant Growth Regulation, 40, 11-19. doi: 10.1007/s00344-020-10069-5.
  12. Nagdalian, A.A., et al. (2023). Effect of selenium nanoparticles on biological and morphofunctional parameters of barley seeds (Nordéum vulgáre L.). Scientific Reports, 13, article number 6453. doi: 10.1038/s41598-023-33581-6.
  13. Park, S., Choi, K.S., Kim, S., Gwon, Y., & Kim, J. (2020). Graphene oxide-assisted promotion of plant growth and stability. Nanomaterials, 10(4), article number 758. doi: 10.3390/nano10040758.
  14. Prylutska, S.V., Tkachenko, T.A., Tkachenko, V.V., & Yemets, A.I. (2024). The role of aquaporins and carbon nanomaterials in abiotic stress in plants. Cytology and Genetics, 58, 428-439. doi: 10.3103/S0095452724050104.
  15. Pykalo, S., Demydov, O., Yurchenko, T., Khomenko, S., Humeniuk, O., Kharchenko, M., & Prokopik, N. (2020). Methods for evaluation of wheat breeding material for drought tolerance. Visnyk of Lviv University. Biological Series, 82, 63-79. doi: 10.30970/vlubs.2020.82.05.
  16. Shoham, J. (2020). The rise of biological products in the crop protection and plant nutrition markets. Outlooks on Pest Management, 31(3), 129-131. doi: 10.1564/v31_jun_09.
  17. Yang, Y., Zhang, R., Zhang, X., Chen, Z., Wang, H., & Li, P.C.H. (2022). Effects of graphene oxide on plant growth: A review. Plants, 11(21), article number 2826. doi: 10.3390/plants11212826.
  18. Yiğit, A., & Chmielewski, F.-M. (2024). A deeper insight into the yield formation of winter and spring barley in relation to weather and climate variability. Agronomy, 14(7), article number 1503. doi: 10.3390/agronomy14071503.
  19. Zhang, X., Cao, H., Zhao, J., Wang, H., Xing, B., Chen, Z., & Li, X., Zhang, J. (2021). Graphene oxide exhibited positive effects on the growth of Aloe vera L. Physiology and Molecular Biology of Plants, 27, 815-824. doi: 10.1007/s12298-021-00979-3.
  20. Zhang, Y., Huang, X., & Huang, K. (2024). Graphene oxide improves the tolerance of Tartary buckwheat to continuous cropping by coordinating the antioxidant defense system and endogenous hormone levels. Plant Stress, 14, article number 100646. doi: 10.1016/j.stress.2024.100646.
  21. Zhao, L., Wang, W., Fu, X., Liu, A., Cao, J., & Liu, J. (2022a). Graphene oxide, a novel nanomaterial as soil water retention agent, dramatically enhances drought stress tolerance in soybean plants. Frontiers in Plant Science, 13, article number 810905. doi: 10.3389/fpls.2022.810905.
  22. Zhao, S., Wang, W., Chen, X., Gao, Y., Wu, X., Ding, M., & Duo, L. (2023). Graphene oxide affected root growth, anatomy, and nutrient uptake in alfalfa. Ecotoxicology and Environmental Safety, 250, article number 114483. doi: 10.1016/j.ecoenv.2022.114483.
  23. Zhao, S., Zhu, X., Mou, M., Wang, Z., & Duo, L. (2022b). Assessment of graphene oxide toxicity on the growth and nutrient levels of white clover (Trifolium repens L.). Ecotoxicology and Environmental Safety, 234, article number 113399. doi: 10.1016/j.ecoenv.2022.113399.