Biological Systems: Theory and Innovation

Antagonistic properties of Bacillus thuringiensis against Liriomiza cicerina Rd

Mykola Lisovyy, V. Chayka
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Abstract

From the corpses of the imago of grain moth the selected strain of bacteria (0376), which on the grounds of spore and crystal formation were referred to the group of  B. thuringiensis. On the 10th day of the experiment the death of the larvae of the Colorado potato beetle (Leptinotarsa decemlineata Say.) when using a strain of B. thuringiensis 0376 accounted for 100% (if deaths in the control to 2.3%). The expediency of cultivation of a strain of  B. thuringiensis 0376 in the volume of the nutrient medium 600ml. Tested the effectiveness of the obtained liquid spore culture on the larvae of Colorado potato beetles. Shown the insecticidal efficacy of B. thuringiensis 0376, which was evident in the number of mines per plant of chickpea – 111,6-152,4 min. per plant, depending on cultivar, whereas in the control variant, the figures were 202,0-342,0 min. studies have shown that a new strain of B. thuringiensis 0376 exhibits substantial biological efficacy in relation to the regulation of the number of gram miner (Liriomiza cicerina Rd.)

Keywords

Bacillus thuringiensis; entomotoxic action; strain; formulation; phytophage; chickpeas

Suggested citation
Lisovyy, M., & Chayka, V. (2022). Antagonistic properties of Bacillus thuringiensis against Liriomiza cicerina Rd. Biological Systems: Theory and Innovation, 13(2), 119-125. https://doi.org/10.31548/biologiya13(3-4).2022.149
References

[1] Bublik, L.I., Vasechko, G.I, &Vasiliev, V.P. (1999). Plant protection guide. Harvest.

[2] Burtseva, L.I. (2001). Methods for the isolation and biotesting of entomopathogenic bacteria. Insect pathogens: Structural and functional aspects. Moscow: All Year Round.

[3] Das, G.P., Magallona, E.D., Raman, K.V., & Adalla, C.B. (1992). Effects of different components of IPM in the management of the potato tuber moth, in storage. Agriculture, Ecosystems & Environment, 41(3–4), 321-325. doi: 10.1016/0167-8809(92)90118-U.

[4] Federici, B.A. (1999). Bacillus thuringiensis in biological control: Principles and Applications of Biological Control. Cambridge: Academic Press.

[5] Fedorov, L.A. (1999). Pesticides – toxic impact on the biosphere and man. Moscow: Science.

[6] Gorbachev, I.V., Grishchenko, V.V., Zakhvatkin, Yu.A., et al. (2002). Plant protection from pests. Moscow: Kolos.

[7] Kandybin, N.V. (1989). Bacterial means of rodent and pest control: Theory and practice. Moscow: Agropromizdat.

[8] Khareba, V.V. (2002). Influence of terms and methods of dictation on seed productivity and quality of seeds of cabbage of a whitehead . Herald of Agrarian Science, 3, 31-32.

[9] Lebedev, S.N. (2002). The use of microbiological preparations in the fight with a moth leafworm in the conditions of the piedmont Crimea. Scientific Works of Scientists of the Crimean State Agrarian University, 75, 43-46.

[10] Leskova, A.Ya., Rybina, L.M., & Stroyeva, I.A. (1984). Identification of cultures Bacillus thuringiensis and assessment of their pathogenic properties. Saint Petersburg: All-Russian Institute of Plant Protection.

[11] Misra, H.S., Khairnar, N.P., Mathur, M., Vijayalakshmi, N., Hire, R.S., Dongre, T.K. & Mahajan, S.K. (2002). Cloning and characterization of an insecticidal crystal protein gene from Bacillus thuringiensis subspecies kenyae. Journal of Genetics, 81(1), 5-11.

[12] Pattya, V.P., Makarenko, N.A., Moklyachuk, L.I., et al. (2005) Agroecological assessment of mineral fertilizers and pesticides. Kyiv: Basis.

[13] Patyka, V.F. (2007). Ecology of Bacillus thuringiensis. Kyiv: Publishing House PDAA.

[14] Secun, M.P., Zherebko, V.M., & Lap, O.M. (2007). Handbook on pesticides. Kyiv: Kolobig.

[15] Slavgorod-Kurpiev, L.E. (2003). Protection of crops from pests and diseases. Donetsk: Donetschyna Publishing House.

[16] World Health Organization & International Programme on Chemical Safety. (‎1999)‎. Microbial pest control agent: Bacillus thuringiensis.
​Retrieved from https://iris.who.int/handle/10665/42242.

[17] Zeddam, J.-L., Soberon, R., Ramos, Z., & Lagnaoui, A. (2003). Producciуn viral y tasas de aplicaciуn del granulovirus usado para el control biolуgico de las polillas de la papa Phthorimaea operculella y Tecia solanivora (Lepidoptera: Gelechiidae). Bol. Sanidad Vegetal Plagas, 29, 659-667. Retrieved from https://www.semanticscholar.org/paper/Producci%C3%B3n-viral-y-tasas-de-aplicaci%C3%B3n-del-usado-el-Soberon-Zeddam/e4a8da369fa004e483019c5829835b67c721fb15.