Biological Systems: Theory and Innovation

Oscillating character of heat and cold shock protein content of oats plants in the conditions of stressful factors of the environment

N. Nesterova, Y. Ruban, O. Pareniuk, K. Shavanova, S. Likar
Download article
Abstract

The article presents the results of research on the gene expression systems of adaptation of sowing oats from the different ecological and geographical origins at low and high temperatures. Specific cold and heat shock proteins were isolated. Significant peroxidase activity was found in Ivory and Neptune varieties, which characterizes their increased resistance to cold oxidative stress. It was found that the varieties Ivory, Arkan and Neptune are resistant to high temperature and water stress, due to the higher content of heat shock proteins with a molecular weight of 80, 70 and 60 kDa. The content of high-molecular proteins (250 kDa) and proteins with a molecular weight of 110, 100, 80 and 70 kDa has decreased in the Hesser variety, which makes it possible to classify this variety as a low-resistance variety. Higher peroxidase activity was found in Ivory, Arkan and Neptune varieties, which characterizes their increased resistance to oxidative stress in winter and summer. It is shown that Ivory and Neptune varieties have high protein expression and are highly frost- and drought-resistant, Arkan and Arthur – moderately frost- and drought-resistant, and Gesser variety has low resistance to stressful environmental conditions, which somewhat limits its use in Ukraine. It is found that the highest peroxidase activity in optimal growing conditions and under the action of water and high temperature stress have varieties Ivory grain purpose and Neptune – fodder purpose, which allows to recommend these varieties for growing in the Forest-Steppe and Steppe of Ukraine

Keywords

sowing oats; varieties; peroxidase; IChF; proline; cold resistance; drought resistance

Suggested citation
Nesterova, N., Ruban, Y., Pareniuk, O., Shavanova, K., & Likar, S. (2022). Oscillating character of heat and cold shock protein content of oats plants in the conditions of stressful factors of the environment. Biological Systems: Theory and Innovation, 13(1), 5-13. https://doi.org/10.31548/biologiya13(1-2).2022.007
References

[1] Illienko, V., Nesterova, N., Pareniuk, O., Ruban, Y., & Shavanova, K. (2017). Estimation of the effect of radionuclide contamination on Vicia sativa L. induction of chlorophyll fluorescence parameters using “Floratest” optical biosensor. Biophotonics-Riga 2017, 10592(20), doi: 10.1117/12.2297563.

[2] Nenko, N., Kisileva, G., Ulianovskaya, E., Yablonskaya, E., & Karavaeva, A. (2018). Physiological-biochemical criteria of the apple-tree resistance to the summer period abiotic stresses. EurAsian Journal of BioSciences, 12(1), 55-61. Retrrieved from https://www.researchgate.net/publication/326842682_Physiological-biochemical_criteria_of_the_apple-tree_resistance_to_the_summer_period_abiotic_stresses.

[3] Nenko, N., Petrov, V.S., Ilina, I.A., Kiseleva, G.K., Sundyreva, M.A., Sokolova, V.V. (2017). Physiological and biochemical mechanisms of adaptation to low-temperature stresses of grapes varieties of various ecological geographical origin. Gardening and Viticulture, 5, 33-38. doi: 10.18454/VSTISP.2017.5.7588.

[4] Nesterova, N., Pareniuk, O., Illienko, V., Ruban, Y., Shavanova, K., and Shpyrka, N. (2019). Physiological reactions in cereals family Avena sativa L. and Avena nuda L., caused by lowerature stress factors, Elnano, 502-506. doi: 10.1109/ELNANO.2019.8783870.

[5] Sarahan, E.V. (2011). Features of the practical application of portable biosensor devices families “Floratest”. Computer Facilities, Networks and Systems, 10, 10-94. Retrieved from http://dspace.nbuv.gov.ua/bitstream/handle/123456789/46457/11-Sarakhan.pdf?sequence=1.

[6] Wan, Y., Schwaninger, H., Li, D., Simon, C.J., Wang, Y., & Zhang, C. (2008). The eco-geographic distribution of wild grape germplasm in China. VITIS – Journal Grapevine Research, 47(2), 77-80. doi: 10.5073/VITIS.2008.47.77-80.

[7] Xu, W., Li, R., Zhang, N., Ma, F., Jiao, Y., Z. Wang. (2014). Transcriptome profiling of Vitis amurensis, an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress. Plant Molecular Biology, 86(4), 527-541. doi: 10.1007/S11103-014-0245-2

[8] Zhuchenko, A.A. (2001). Adaptive system of plant breeding (ecological and genetic basis). Moscow: Agrorus.