Biological Systems: Theory and Innovation

The study presents the results of obtaining regenerated plants of asparagus from seeds. Surface sterilizing the seeds by 0,75% sodium hypochlorite for 30 min is effective; during this obtained 83% viable sterile plants. The Murashige and Skoog medium supplemented with 6-benzylaminopurine (2 mg/L), inositol (100 mg/L), and thiamine (0.4 mg/L) was found to be the best for seed germination. The expediency of using kinetin (1 mg/L) as a growth regulator to obtain a homogeneous plant material was established. The reproduction coefficient was 6.0. Only 11% of the explants formed callus. For the selection needs and production of somaclonal variants, the use of the culture medium with indole-3-acetic acid (0,2 mg/L) and 6-benzylaminopurine (1 mg/L) is justified. In this condition, reproduction coefficient was 3.7, and the level of different intensity callusogenesis was 59%. The rooting of obtained plants was performed in Murashige and Skoog medium supplemented with a half dose of macro- and micronutrients and growth regulators. Rooting frequency was up to 63%. The knowledge of hormonal requirements helps to promote isolated tissue and cells technologies of asparagus with purpose of rapid propagation and obtaining healthy, high-quality planting material

Asparagus officinalis; in vitro; seeds; sterilization; morphogenesis; cytokinin; auxin